smear examination and cornea scraping culture. Histopathologi-cal examination findings were also recorded if diseased corneal tissues were obtained at the time of surgery. After instilling a topical local anesthetic (lignocaine hydro-chloride 4% drops), the extraocular environment was sterilized with 5% povidone iodine solution, and scrapings from the affect-
1. Cornea. 2002 Oct;21(7):643-7. Evaluation of corneal scraping smear examination methods in the diagnosis of bacterial and fungal keratitis: a survey of eight years of laboratory experience.
smear examination and cornea scraping culture. Histopathologi-cal examination findings were also recorded if diseased corneal tissues were obtained at the time of surgery. After instilling a topical local anesthetic (lignocaine hydro-chloride 4% drops), the extraocular environment was sterilized with 5% povidone iodine solution, and scrapings from the affect-
Scraping of cornea for smear or culture information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
corneal scrape and making a diagnosis. However, there are settings in which there are either limited or no laboratory facilities . Taking a corneal scrape What you will need: • Making a diagnosis 21-guage needles or Kimura scalpel • 2x clean microscope slides • 1x blood agar plate (FBa) • 1x Sabouraud glucose agar plate (SGa) • 1x brain heart infusion broth (BHI) (for . • Gently smear material on the surface of agar in C-streaks (Figure 3); taking care not to
chocolate agar plate making one row of “C†formations for each scraping. c. Corneal scraping for smear preparation may be obtained. 1) Label 4 clean glass slides with the patient name and date of birth. Label two . Corneal scraping – submit corneal scraping in 1mL of sterile saline in a screw-capped, sterile container. Swabs are not acceptable.
Correlation of Corneal Scraping Smear Examination and Presence of Hypopyon to Microbial Culture Results in Suspected Infectious Keratitis
.t returned to the clinic on the ninth day after his initial visit, he was still continuously wearing the DSCL. Slit-lamp examination showed that there was an enlarged deep corneal stromal infiltrate (Figure 2).
Evaluation of corneal scraping smear examination methods in the diagnosis of bacterial and fungal keratitis: a survey of eight years of laboratory experien
This article aims to provide a comprehensive guide to taking a corneal scrape and making a diagnosis.